Development of a Novel, Sensitive, Selective, and Fast Methodology to Determine Malondialdehyde in Leaves of Melon Plants by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry

Table 2

Recovery study and analytical performance of the proposed SPE-UPLC-(+)ESI-MS/MS methodology for the MDA determination in melon leaves.

MDA added (µg·L^−1)	MDA found (µg·L^−1)			Precision (RSD %)				Accuracy (% recovery)
	Day 1 ()	Day 2 ()	Day 3 ()	Day 1	Day 2	Day 3	Interday	Day 1	Day 2	Day 3
0	425 ± 11	414 ± 12	442 ± 11	2.6	2.9	2.5	3.3	—	—	—
200	631 ± 18	639 ± 19	680 ± 20	2.8	3.0	2.9	4.0	101	104	106
300	747 ± 21	707 ± 19	691 ± 17	2.8	2.7	2.5	4.0	103	99	93
500	869 ± 23	886 ± 24	942 ± 26	2.6	2.7	2.8	4.2	94	97	100
1000	1425 ± 35	1357 ± 33	1341 ± 33	2.5	2.4	2.5	3.7	100	96	93

Values are means ± standard deviation.