UPLC-Tandem Mass Spectrometry Method for Simultaneous Determination of Fluoxetine, Risperidone, and Its Active Metabolite 9-Hydroxyrisperidone in Plasma: Application to Pharmacokinetics Study in Rats

Table 2

Intraday and interday precision and accuracy of FLX, RIS, and 9- OH-RIS in rat plasma.

Nominal conc. (ng/ml)	Intraday reproducibility			Interday reproducibility
	Mean ± SD	Precision (% CV)	Accuracy (%)	Mean ± SD	Precision (% CV)	Accuracy (%)
FLX
1.0	1.13 ± 0.24	17.94	113.98	1.18 ± 0.19	16.09	118.16
100.0	88.05 ± 10.06	13.45	88.05	82.59 ± 10.79	13.06	82.59
1000.0	971.16 ± 22.92	2.46	97.12	977.51 ± 29.49	3.02	97.75
RIS
0.2	0.22 ± 0.02	11.16	109.5	0.21 ± 0.03	15.2	103.64
100.0	92.05 ± 11.77	12.79	92.05	96.44 ± 13.52	14.02	96.44
1000.0	1031.92 ± 3907	3.79	103.19	1028.48 ± 32.57	3.17	102.8
9-OH-RIS
0.2	0.24 ± 0.02	9.46	120.7	0.22 ± 0.04	19.36	109.6
100.0	90.47 ± 3.97	4.39	90.47	92.81 ± 5.59	6.03	92.81
1000.0	981.79 ± 34.05	3.47	98.18	962.03 ± 31.87	3.31	96.2