Research Article
Development of a LC-MS/MS Method for the Simultaneous Detection of Tricarboxylic Acid Cycle Intermediates in a Range of Biological Matrices
Table 1
LC-MS/MS parameters of each analyte.
| | Analyte | Retention time (min) | Precursor ion (m/z) | Product ion (m/z) | Collision energy | Cell accelerator voltage | Polarity |
| | D4-citric acid | 1.13 | 195 | 114 | 12 | 4 | Negative | | D4-citric acid | 1.13 | 195 | 177 | 10 | 4 | Negative | | Isocitric acid | 0.85 | 191 | 155 | 10 | 5 | Negative | | Citric acid | 1.13 | 191 | 111 | 10 | 5 | Negative | | Citric acid | 1.13 | 191 | 87 | 18 | 5 | Negative | | Glutamic acid | 0.6 | 148 | 130 | 8 | 4 | Positive | | Glutamic acid | 0.6 | 148 | 84 | 12 | 4 | Positive | | Malic acid | 0.7 | 133 | 115 | 10 | 5 | Negative | | Malic acid | 0.7 | 133 | 71 | 14 | 5 | Negative | | Succinic acid | 1.6 | 117 | 99 | 10 | 5 | Negative | | Succinic acid | 1.6 | 117 | 73 | 10 | 5 | Negative | | Fumaric acid | 1.3 | 115 | 71 | 10 | 5 | Negative | | Fumaric acid | 1.3 | 115 | 41 | 18 | 5 | Negative | | Lactic acid | 1.05 | 89 | 43 | 10 | 4 | Negative | | α-Ketoglutaric acid | 1.03 | 145 | 101 | 10 | 5 | Negative | | α-Ketoglutaric acid | 1.03 | 145 | 57 | 10 | 5 | Negative | | Oxaloacetic acid | 0.58 | 131 | 87 | 10 | 5 | Negative | | Oxaloacetic acid | 0.58 | 131 | 41 | 54 | 5 | Negative |
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