Research Article
Development of a LC-MS/MS Method for the Simultaneous Detection of Tricarboxylic Acid Cycle Intermediates in a Range of Biological Matrices
Table 2
Validation data for each analyte in human plasma.
| | Analyte | Linearity range (µM) | | Precision intraday (%)
() | Precision interday (%)
() | LOD | LOQ |
| | Citric acid | 0–520 | 0.9998 | 4.9 | 6.3 | 0.06 | 0.18 | | Isocitric acid | 0–520 | 0.9997 | 4.6 | 10.5 | 0.06 | 0.18 | | Malic acid | 0–372 | 0.9999 | 8.9 | 13.5 | 0.06 | 0.18 | | Lactic acid | 0–1100 | 0.9987 | 4.44 | 9.9 | 1 | 3 | | Succinic acid | 0–840 | 0.9998 | 8.1 | 18.9 | 0.06 | 0.18 | | Fumaric acid | 0–430 | 0.9998 | 8.8 | 12.2 | 1 | 3 | | Glutamic acid | 0–340 | 0.9998 | 7.4 | 12.6 | 0.05 | 0.15 |
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Values in µM for 1 : 10 sample dilution.
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