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Journal of Analytical Methods in Chemistry
Volume 2017 (2017), Article ID 6354532, 7 pages
Research Article

Chemical Characterization and Biological Activities of Essential Oil Obtained from Mint Timija Cultivated under Mineral and Biological Fertilizers

1Faculté des Sciences Semlalia, Université Cadi Ayyad, Marrakech, Morocco
2Ecole Normale Supérieure de Tétouan, Université Abdelmalek Essaadi, Tétouan, Morocco
3School of Science and Health, Western Sydney University, Locked Bag 1797, Penrith, NSW 2751, Australia
4Université de Lyon, 69622 Lyon, France
5Université Lyon 1, Villeurbanne, France
6CNRS, UMR5557, Ecologie Microbienne, Villeurbanne, France
7INRA, UMR1418, Villeurbanne, France

Correspondence should be addressed to Ayoub Kasrati

Received 20 July 2017; Accepted 3 October 2017; Published 12 November 2017

Academic Editor: Boryana M. Nikolova-Damyanova

Copyright © 2017 Ayoub Kasrati et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Cultivation of mint timija (Mentha suaveolens subsp. timija (Briq.) Harley) constitutes a promising solution to the conservation and sustainable utilization of this Moroccan endemic and threatened species. Optimized agronomic practices require mineral and/or biological fertilizer applications. The aim of this study was to determine the effects of application of a complete (N, P, and K) mineral fertilizer and vesicular arbuscular mycorrhizae (VAM) inoculation on the composition, antioxidant, and insecticidal properties of mint timija essential oils (EOs). The GC-MS analyses identified 27 components representing more than 99.9% of the total oils. Menthone (40.7–49.3%), pulegone (31.3–36.5%), and isomenthone (2.5–4.4%) were found to be the main constituents. Cultivation of mint timija with mineral fertilizer and VAM inoculation induced an increase in menthone content and a parallel decrease of pulegone. Both treatments enhanced the antioxidant activity of the investigated EOs in all assays (IC50 ranged from 2.34 ± 0.03 mg/mL to 6.82 ± 0.25 mg/mL), while no significant difference in the toxicities of these oils against Tribolium confusum du Val. has been observed. Overall, we conclude that cultivation using complete mineral fertilizer and VAM inoculation could be useful in modulating the chemical composition and enhancing the antioxidant activity of the EO of this endemic Moroccan species.