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Journal of Analytical Methods in Chemistry
Volume 2017, Article ID 7157953, 7 pages
Research Article

Development of an Analytical Protocol for Determination of Cyanide in Human Biological Samples Based on Application of Ion Chromatography with Pulsed Amperometric Detection

1Department of Analytical Chemistry, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza Str. 11/12, 80-233 Gdańsk, Poland
2Faculty of Earth Sciences, University of Silesia, Będzińska 60, 41-200 Sosnowiec, Poland

Correspondence should be addressed to Żaneta Polkowska;

Received 19 April 2017; Revised 10 July 2017; Accepted 2 August 2017; Published 21 November 2017

Academic Editor: Hana Sklenarova

Copyright © 2017 Ewa Jaszczak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A simple and accurate ion chromatography (IC) method with pulsed amperometric detection (PAD) was proposed for the determination of cyanide ion in urine, sweat, and saliva samples. The sample pretreatment relies on alkaline digestion and application of Dionex OnGuard II H cartridge. Under the optimized conditions, the method showed good linearity in the range of 1–100 μg/L for urine, 5–100 μg/L for saliva, and 3–100 μg/L for sweat samples with determination coefficients . Low detection limits (LODs) in the range of 1.8 μg/L, 5.1 μg/L, and 5.8 μg/L for urine, saliva, and sweat samples, respectively, and good repeatability (CV < 3%, ) were obtained. The proposed method has been successfully applied to the analysis of human biological samples.