Determination of Epimedin B in Rat Plasma and Tissue by LC-MS/MS: Application in Pharmacokinetic and Tissue Distribution Studies

Table 2

Intra- and interday accuracy and precision for the determination of epimedin B in plasma and tissue samples (, 6 replicates per day for 3 days).

Matrix	Conc.; ng/mL	Interday; RSD (%)	Intraday; RSD (%)
Plasma	2	5.528	8.673
	20	12.91	3.314
	400	1.250	3.853
Liver	2	6.264	5.039
	20	1.890	3.058
	400	3.507	1.333
Heart	2	2.216	4.701
	20	1.175	1.755
	400	1.609	1.117
Spleen	2	7.750	6.267
	20	3.824	1.733
	400	6.551	2.624
Lung	2	11.72	4.247
	20	4.767	3.324
	400	7.277	1.714
Kidney	2	14.95	4.529
	20	3.984	2.235
	400	4.209	1.799
Brain	2	10.36	6.442
	20	13.42	2.758
	400	10.25	1.678
Ovary	2	6.264	5.039
	20	1.890	3.058
	400	3.507	1.333
Womb	2	6.264	5.039
	20	1.890	3.058
	400	3.507	1.333