Chromatographic Characterization and Method Development for Determination of Levetiracetam in Saliva: Application to Correlation with Plasma Levels

Figure 3

Chromatograms for saliva samples spiked with DEBA (internal standard, IS) and decreasing levels of LVT according to the direction of the dashed arrow (36, 28, 4, and zero µg/ml). Note the overlapping with the peak of IS and at about 5.8 min. In the sample spiked with 28 µg/ml of LVT no DEBA was added. Column: Synergi Polar RP (150 mm × 4.6 i.d). Mobile phase was 3.5% ACN in phosphate buffer at pH 6 with a flow rate of 1.5 ml/min.