The Elevation of LC-ESI-Q-TOF-MS Response in the Analysis of Isoquinoline Alkaloids from Some Papaveraceae and Berberidaceae Representatives

Table 4

The behaviour of model alkaloids in different collision energies together with the abundance of obtained signals.

Number	Alkaloid	Precursor ion	Major fragments obtained at various CID voltage (V) with abundances in different intensities (%)
(1)	Berberine	336	10 V	336 (100), 337 (27), 321 (5)
			20 V	336 (100), 321 (43), 292 (22)
			30 V	320 (100), 292 (73), 321 (69), 306 (26)
			40 V	320 (100), 278 (55), 292 (49), 318 (31), 306 (25)
			50 V	278 (100), 318 (38), 292 (31), 279 (2)
			60 V	278 (100), 263 (31), 249 (27), 220 (26)
			80 V	191 (100), 220 (47), 204 (46), 192 (38)
(2)	Magnoflorine	342	10 V	342 (100), 343 (31), 297 (12), 58 (10)
			20 V	297 (100), 265 (85), 342 (78), 58 (71)
			30 V	58 (100), 265 (29), 297 (16)
			40 V	58 (100), 191 (31), 222 (29)
			50 V	58 (100), 179 (20), 192 (10)
			60 V	58 (100), 165 (56), 176 (20)
			80 V	58 (100), 165 (50), 178 (40)
(3)	Sanguinarine	332	10 V	332 (100), 333 (27), 334 (3)
			20 V	332 (100), 333 (24), 304 (7)
			30 V	332 (100), 274 (41), 304 (39), 317 (34)
			40 V	274 (100), 304 (60), 246 (52), 216 (30)
			50 V	304 (100), 216 (55), 246 (48), 189 (22)
			60 V	246 (100), 216 (60), 260 (40), 189 (30)
			80 V	189 (100), 202 (85), 176 (75)
(4)	Berbamine	609	10 V	609 (100), 610 (38), 611 (35), 612 (16)
			20 V	609 (100), 611 (76), 206 (29), 579 (12)
			30 V	609 (100), 611 (80), 192 (80), 578 (69), 206 (64), 296 (45)
			40 V	206 (100), 192 (93), 174 (36), 190 (34), 296 (34)
			50 V	206 (100), 192 (90), 296 (52), 239 (41)
			60 V	206 (100), 192 (62), 174 (47), 296 (40)
			80 V	160 (100), 191 (94), 165 (85), 174 (84)

Note: the most optimal parameters are given in bold.