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Journal of Analytical Methods in Chemistry
Volume 2017, Article ID 8704639, 9 pages
Research Article

Magnetic Solid-Phase Extraction Using Fe3O4@SiO2 Magnetic Nanoparticles Followed by UV-Vis Spectrometry for Determination of Paraquat in Plasma and Urine Samples

1Analysis and Test Center of Jiangsu Marine Resources Development Research Institute, Huaihai Institute of Technology, Lianyungang, China
2School of Chemistry and Chemical Engineering, Huaihai Institute of Technology, Lianyungang, China
3Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Huaihai Institute of Technology, Lianyungang, China
4The First People’s Hospital of Lianyungang City, Lianyungang, China

Correspondence should be addressed to Xiaobing Chen; moc.361@1097mxj

Received 11 March 2017; Accepted 11 June 2017; Published 17 July 2017

Academic Editor: Krystyna Pyrzynska

Copyright © 2017 Ou Sha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A rapid and simple method was optimized and validated for the separation and quantification of paraquat, a frequently used herbicide and a leading cause of fatal poisoning worldwide, at trace levels with UV-Vis spectrophotometry in plasma and urine samples by direct magnetic solid-phase extraction. Fe3O4@SiO2 nanoparticles (NPs) were used as the magnetic solid-phase extraction agents and the paraquat absorbed on NPs was eluted using NaOH and ascorbic acid. Upon optimization, paraquat could be extracted and concentrated from various samples by 35-fold. The linear range, limit of detection (LOD), correlation coefficient (), and relative standard deviation (RSD) could reach 15.0–400.0 μg/L, 12.2 μg/L, 0.9987, and 0.65% (, = 40.0 μg/L), respectively. The Fe3O4@SiO2 NPs could be reused up to five times. The method was successfully applied to the determination of paraquat in urine and plasma at different hemoperfusion numbers in a local hospital for the patient of paraquat poisoning. The experiment result could not only enable immediate medical intervention but also benefit patients’ survival.