Research Article
Comparative Liquid Chromatographic Study for Concurrent Determination of Canagliflozin and Metformin in Combined Tablets
Table 1
Comparison between different LC-UV reported methods and the proposed methods for simultaneous determination of CANA and MET.
| | Methods | Column | Mobile phase | Run time (min) | Sensitivity |
| | Reported method [7] | C18 column
(250 mm × 4.6 mm, 5 μm)
At ambient temperature | Ammonium acetate : acetonitrile
(pH 3.5) (65 : 35, v/v) | 8 min | CANA (5–30 μg·mL−1)
MET (50–300 μg·mL−1) |
| | Reported method [8] | C18 column
(250 mm × 4.6 mm, 5 μm)
At Temperature at 30°C | Phosphate buffer : acetonitrile
(pH 4.5) (65 : 35, v/v) | 4 min | CANA (5–30 μg·mL−1)
MET (50–300 μg·mL−1) |
| | Reported method [9] | ODS column
(250 mm × 4.6 mm, 5µm)
At Temperature at 30°C | Phosphate buffer : acetonitrile : methanol (pH 4.5) (40 : 40 : 20, v/v) | 7 min | CANA (5–30 μg·mL−1)
MET (50–300 μg·mL−1) |
| | Proposed method (A) | C18 column
(100 mm × 2.1 mm, 3 μm)
At ambient temperature | Methanol : phosphate buffer
(pH 3.2) (75 : 25, v/v) | 1.1 min | CANA (1–50 μg·mL−1)
MET (0.5–100 μg·mL−1) |
| | Proposed method (B) | Hypersil gold
(50 mm × 2.1 mm, 1.9 μm)
At ambient temperature | Methanol : phosphate buffer
(pH 3.5) (80 : 20 v/v) | 1 min | CANA (0.1–50 μg·mL−1)
MET (0.25–100 μg·mL−1) |
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