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Journal of Analytical Methods in Chemistry
Volume 2017 (2017), Article ID 9528472, 9 pages
Research Article

SPE/TLC/Densitometric Quantification of Selected Synthetic Food Dyes in Liquid Foodstuffs and Pharmaceutical Preparations

Department of Analytical Chemistry, Medical University of Lodz, Ul. Muszynskiego 1, 90-151 Lodz, Poland

Correspondence should be addressed to Anna W. Sobańska

Received 28 April 2017; Accepted 18 June 2017; Published 17 July 2017

Academic Editor: Ricardo Jorgensen Cassella

Copyright © 2017 Anna W. Sobańska et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Selected synthetic food dyes (tartrazine, Ponceau 4R, Brilliant Blue, orange yellow, and azorubine) were isolated from liquid preparations (mouthwashes and beverages) by Solid Phase Extraction on aminopropyl-bonded silica with diluted aqueous sodium hydroxide as an eluent. The extraction step was followed by thin layer chromatography on silica gel 60 with chloroform-isopropanol-25% aq. ammonia 1 : 3 : 1 (v/v/v) as mobile phase and the densitometric quantification of dyes was achieved using quadratic calibration plots (; = 0.04–0.09 μgspot−1). The overall recoveries for all studied dyes were at the average level of over 90% and the repeatability of the proposed procedure (CV ≤ 4.1%) was sufficient to recommend it for the routine quantification of the aforementioned dyes in liquid matrices.