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Journal of Analytical Methods in Chemistry
Volume 2017 (2017), Article ID 9812894, 8 pages
https://doi.org/10.1155/2017/9812894
Research Article

A New Kinetic Spectrophotometric Method for the Quantitation of Amorolfine

1Departamento de Química Analítica e Inorgánica, Facultad de Ciencias Químicas, Universidad de Concepción, Concepción, Chile
2Laboratorio de Química Analítica, Facultad de Ciencias, Universidad de Chile, Santiago, Chile

Correspondence should be addressed to C├ęsar Soto; lc.cedu@otosrasec

Received 10 October 2016; Revised 21 December 2016; Accepted 15 January 2017; Published 28 February 2017

Academic Editor: Christos Kontoyannis

Copyright © 2017 César Soto et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Amorolfine (AOF) is a compound with fungicide activity based on the dual inhibition of growth of the fungal cell membrane, the biosynthesis and accumulation of sterols, and the reduction of ergosterol. In this work a sensitive kinetic and spectrophotometric method for the AOF quantitation based on the AOF oxidation by means of KMnO4 at 30 min (fixed time), pH alkaline, and ionic strength controlled was developed. Measurements of changes in absorbance at 610 nm were used as criterion of the oxidation progress. In order to maximize the sensitivity, different experimental reaction parameters were carefully studied via factorial screening and optimized by multivariate method. The linearity, intraday, and interday assay precision and accuracy were determined. The absorbance-concentration plot corresponding to tap water spiked samples was rectilinear, over the range of 7.56 × 10−6–3.22 × 10−5 mol L−1, with detection and quantitation limits of 2.49 × 10−6 mol L−1 and 7.56 × 10−6 mol L−1, respectively. The proposed method was successfully validated for the application of the determination of the drug in the spiked tap water samples and the percentage recoveries were 94.0–105.0%. The method is simple and does not require expensive instruments or complicated extraction steps of the reaction product.