A Standardized Approach to Quantitative Analysis of Nicotine in e-Liquids Based on Peak Purity Criteria Using High-Performance Liquid Chromatography

Table 8

Robustness.

Organic phase composition		Sample	Peak area	USP resolution	Purity angle	Purity threshold
% mobile phase B	% mobile phase C
26	14	e-Liquid flavor V	1031106	1.46	0.729	1.159
			1031686	1.51	0.390	1.106
		e-Liquid flavor W	1023000	1.48	0.984	1.138
			1021702	1.48	0.915	1.144
28	12	e-Liquid flavor V	1030040	2.31	0.192	1.063
			1026349	2.36	0.471	1.104
		e-Liquid flavor W	1023658	2.30	0.134	1.093
			1027008	2.28	0.295	1.128