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Journal of Analytical Methods in Chemistry
Volume 2018, Article ID 1794650, 8 pages
Research Article

Nonvolatile Chemical Constituents from the Leaves of Ligusticopsis wallichiana (DC.) Pimenov & Kljuykov and Their Free Radical-Scavenging Activity

1Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo ku, Kumamoto 862-0973, Japan
2Program for Leading Graduate Schools, Health Life Science: Interdisciplinary and Glocal Oriented (HIGO) Program, Kumamoto University, Kumamoto, Japan

Correspondence should be addressed to Hari Prasad Devkota;

Received 6 October 2017; Revised 5 December 2017; Accepted 25 December 2017; Published 1 February 2018

Academic Editor: Eduardo Dellacassa

Copyright © 2018 Hari Prasad Devkota et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Different plant parts of Ligusticopsis wallichiana (family: Apiaceae) are widely used as traditional medicines. Although many volatile constituents are already identified from the leaves of L. wallichiana, there is no detailed report on the nonvolatile constituents. In the present study, we aimed to isolate and identify the major chemical constituents from the leaves. Bhutkesoside A (1), falcarindiol (2), ferulic acid (3), cnidioside A (4), quercetin 3-O-β-D-glucopyranoside (5), rutin (6), 4′-O-methylquercetin 3-O-β-D-glucopyranoside (7), scopoletin (8), umbelliferone (9), eugenol 4-O-β-D-glucopyranoside (10) and pumilaside A (11) were isolated from the 70% MeOH extract. The structures of isolated compounds were elucidated on the basis of 1H- and 13C-NMR spectroscopic data. Compounds 4–11 are reported for the first time from L. wallichiana. Compounds 5 and 6 showed potent free radical-scavenging activity.