Research Article

Design of Amperometric Biosensors for the Detection of Glucose Prepared by Immobilization of Glucose Oxidase on Conducting (Poly)Thiophene Films

Table 1

Selected validation data for the determination of glucose with (a) poly(2,2′-BT)/GOx and (b) poly(4,4′-bBT)/GOx-based biosensors.

BiosensorLoD (μM)LoQ (μM)Linearity range (mM), Y = aX + b; R2Response time (s; C glucose, mM)Intermediate precision (CV; C glucose, mM)Stability (days)Trueness, recovery (% ± SD)

Poly(2,2′-BT)/GOx30900.09–5.20
a = 1.5 × 10−3± 0.2 × 10−3
b = 5 × 10−7± 6 × 10−7
R2 = 0.999
180; 0.2
120; 1
7.0; 1>1593 ± 6

Poly(4,4′-bBT)/GOx501500.15–5.20
a = 3.4 × 10−4± 0.1 × 10−4
b = 4 × 10−8± 7 × 10−8
R2 = 0.997
50; 0.2
20; 1
2.8; 1>30101 ± 4