Development and Validation of an HPLC Method for Determination of Antidiabetic Drug Alogliptin Benzoate in Bulk and Tablets

Table 1

Results of method optimization.

Column used	Mobile phase	Flow rate	Wavelength	Observation	Result
Restek C18, 125 × 4.0 mm i.d., 5 µm	(Buffer : methanol) (45 : 55) v/v	1.0 ml/min	216 nm	Poor resolution 1.4	Method rejected
Thermo Scientific C18, 250 × 4.6 mm i.d., 5 µm	(Buffer : acetonitrile) (25 : 75) v/v	1.0 ml/min	277 nm	Poor resolution 1.6	Method rejected
Thermo Scientific C18, 250 × 4.6 mm i.d., 5 µm	(Buffer : acetonitrile) (45 : 55) v/v	1.0 ml/min	277 nm	Good resolution 2.4	Method accepted