Journal of Analytical Methods in Chemistry / 2018 / Article / Tab 1

Research Article

Development and Validation of an HPLC Method for Determination of Antidiabetic Drug Alogliptin Benzoate in Bulk and Tablets

Table 1

Results of method optimization.

Column usedMobile phaseFlow rateWavelengthObservationResult

Restek C18, 125 × 4.0 mm i.d., 5 µm(Buffer : methanol) (45 : 55) v/v1.0 ml/min216 nmPoor resolution 1.4Method rejected
Thermo Scientific C18, 250 × 4.6 mm i.d., 5 µm(Buffer : acetonitrile) (25 : 75) v/v1.0 ml/min277 nmPoor resolution 1.6Method rejected
Thermo Scientific C18, 250 × 4.6 mm i.d., 5 µm(Buffer : acetonitrile) (45 : 55) v/v1.0 ml/min277 nmGood resolution 2.4Method accepted

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