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Journal of Analytical Methods in Chemistry
Volume 2018, Article ID 1981436, 9 pages
Research Article

Rapid and Simple Determination of Sarafloxacin and Difloxacin in Beef by Capillary Electrophoresis Coupled with Solid-Phase Extraction

1Department of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China
2College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China
3Department of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, China
4Tianjin Engineering and Technology Research Center of Agricultural Products Processing, Tianjin 300384, China

Correspondence should be addressed to Shijie Yan; moc.621@eijihsnay

Received 1 January 2017; Revised 20 February 2017; Accepted 7 March 2017; Published 20 February 2018

Academic Editor: Pablo Richter

Copyright © 2018 Qian Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A simple and rapid capillary electrophoresis method with diode array detector was developed for determination of sarafloxacin and difloxacin in beef. In this study, the experimental parameters affecting the determination were systematically optimized, including wavelength, buffer system, pH and concentration, and separation temperature and voltage. Under the optimal conditions, sarafloxacin and difloxacin could be quantified within 4 min using H3BO3/Na2B4O7 buffer (35 mmol/L, pH 8.8) as background electrolyte, 25 kV as separation voltage, and 22°C as the column temperature. The linear range of the method was 1–20 μg/mL with LOD 0.8 μg/mL for sarafloxacin and 0.5–20 μg/mL with LOD 0.3 μg/mL for difloxacin. The RSDs for the peak area of 8 μg/mL sarafloxacin were 4.8% (intraday) and 7.8% (interday), respectively. The proposed method has been applied to determine the residue of sarafloxacin and difloxacin in beef samples with the satisfactory recovery.