Journal of Analytical Methods in Chemistry

Research Article

Synchronized Survey Scan Approach Allows for Efficient Discrimination of Isomeric and Isobaric Compounds during LC-MS/MS Analyses

MRM transitions and MRM-dependent product ion scan of isomeric and isobaric compounds from different sample types.


Sample type	Compound	Retention time (Rt) (min)	Precursor (m/z)	Optimal transitions	Collision energy (eV)	MRM-dependent product ion

Standard	1,3-O-dicaffeoylquinic acid	13.71	515	515 > 353	18	515→353, 335, 191 (bp), 179, 161, 135
				515 > 179/191	28/40
	3,4-O-dicaffeoylquinic acid	21.34	515	515 > 179/353	28/18	515→353, 335, 191, 179, 173 (bp), 161, 135
				515 > 191	40
	3,5-O-dicaffeoylquinic acid	22.25	515	515 > 353	18	515→353, 191 (bp), 179, 135
				515 > 191/179	40/28
	1,5-O-dicaffeoylquinic acid	22.82	515	515 > 191	40	515→353, 191 (bp)
				515 > 353	18
	4,5-O-dicaffeoylquinic acid	24.75	515	515 > 353	18	515→353 (bp), 191, 179, 173, 135
				515 > 179	28
Bidens pilosa	3,4-O-dicaffeoylquinic acid	21.24	515	515 > 179/353	28/18	515→353, 335, 191, 179, 173 (bp), 161, 135
				515 > 191	40
	3,5-O-dicaffeoylquinic acid	22.05	515	515 > 353	18	515→353, 191 (bp), 179, 135
				515 > 191/179	40/28
	4,5-O-dicaffeoylquinic acid	24.61	515	515 > 353	18	515→353 (bp), 191, 179, 173, 135
				515 > 179	28
Moringa oleifera	3-O-(4′-O-caffeoyl glucosyl) quinic acid	4.89	515	515 > 179	28	515→353, 341, 191, 179 (bp)
				—	—
	4-O-(4′-O-caffeoyl glucosyl) quinic acid	7.32	515	515 > 179	28	515→353, 341, 191, 179, 173 (bp)
				515 > 353	18