Research Article

HPLC Method for Quantification of Caffeine and Its Three Major Metabolites in Human Plasma Using Fetal Bovine Serum Matrix to Evaluate Prenatal Drug Exposure

Table 2

Results of intra- and interday variability during validation of the HPLC method. Accuracy is expressed as percentage of the nominal value. Precision is expressed in terms of the percentage of the coefficient of variation.

Concentration (µg/mL)Interday, , accuracy (%)/precision (CV)Intraday, , accuracy (%)/precision (CV)
ThebParTheoCafThebParTheoCaf

0.5103.7 (8.8)96.6 (4.0)96.5 (2.3)100.73 (8.0)101.2 (8.7)97.1 (3.5)97.5 (4.1)98.4 (6.3)
5100.9 (5.7)103.2 (4.9)99.0 (4.6)102.2 (6.6)100.6 (6.8)102.1 (5.0)99.9 (5.0)103.4 (5.6)
30101.9 (7.5)102.1 (6.4)103.2 (5.5)105.2 (5.7)102.9 (8.7)101.0 (5.5)103.0 (5.4)106.2 (4.8)

Interday results are the accumulation of determination of 3 quality control samples in three different days for each concentration level.