Research Article
Further Validation of a Rapid Screening Semiquantitative Thin-Layer Chromatographic Method for Marketed Antimalarial Medicines for Adoption in Malawi
Table 2
Solvent systems for antimalarial active pharmaceutical ingredients.
| | API | Solvent system 1 (S1) | Solvent system 2 (S2) | Spraying reagent | Colour |
| | Artesunate | Ethanol:ammonia (100 : 0.5) | Ethanol:toluene:ammonia (70 : 30 : 1.5) | Anisaldehyde/methanol | Purple | | Artemether | Petrol:ethyl acetate (70 : 30) | Toluene:ethyl acetate (70 : 30) | Anisaldehyde/methanol | Purple | | Artenimol | Toluene:ethyl acetate (60 : 40) | Toluene:ethyl acetate (70 : 30) | Anisaldehyde/methanol | Purple | | Quinine | Methanol:ammonia (100 : 1.5) | Ethyl acetate:acetic acid:water (60 : 20 : 20) | I2/KI | Brown | | Pyrimethamine | Ethyl acetate:methanol:ammonia (80 : 15:5) | Ethyl acetate:acetic acid:water (60 : 20 : 20) | I2/KI | Brown | | Sulphadoxine | Ethyl acetate:methanol:ammonia (80 : 15:5) | Ethyl acetate:methanol:acetic acid (75 : 25 : 1) | I2/KI | Brown | | Lumefantrine | Ethyl acetate:acetic acid:toluene (4 : 2 : 18) | Ethyl acetate:acetic acid (10 : 5) | I2/KI | Brown |
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