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Journal of Analytical Methods in Chemistry
Volume 2018, Article ID 2434691, 9 pages
https://doi.org/10.1155/2018/2434691
Research Article

Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material

1Department of Drug Analysis, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, Belgrade 11221, Serbia
2Chair for Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Aškerčeva cesta 7, Ljubljana 1000, Slovenia
3Jožef Stefan Institute, Jamova 39, Ljubljana 1000, Slovenia

Correspondence should be addressed to Jelena Golubović; sr.ca.gb.ycamrahp@bulog

Received 28 August 2017; Revised 30 November 2017; Accepted 11 December 2017; Published 20 February 2018

Academic Editor: Pablo Richter

Copyright © 2018 Nevena Maljurić et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Diabetes mellitus is one of the leading world’s public health problems. Therefore, it is of a huge interest to develop new antidiabetic drugs. Apart from traditional therapy of diabetes, nowadays, importance is given to natural substances with antidiabetic potential. Fomes fomentarius is a mushroom widely used for different purposes, due to its range of already confirmed activities. Fomentariol is a constituent of Fomes fomentarius, responsible for its antidiabetic potential. In that respect, it is important to develop a method for isolation and quantification of fomentariol from fungal material, which will be simple and efficient. Multistep, complex extraction applied in the previously reported studies was avoided with ethanol, providing rapid single-step extraction. The presence of fomentariol in ethanolic extract was confirmed by high-resolution mass spectrometry. Semipreparative HPLC method was developed and applied for isolation from ethanol extract and purification of the active compound fomentariol. It was a gradient reversed-phase method with a mobile phase consisting of acetonitrile and 0.1% formic acid in water and total run time of 15 minutes. The amount of 6.5 mg of high-purity fomentariol was determined by quantitative NMR with toluene as internal standard. The isolated and determined amount of substance can be further used for the quantitative estimation of activity of fomentariol.