Development of an Enzyme-Linked Immunosorbent Assay and Gold-Labelled Immunochromatographic Strip Assay for the Detection of Ancient Wool

Figure 4

ELISA titration results in different conditions. (a) The dilution ratio of anti-keratin primary antibody is 1 : 200, 1 : 500, 1 : 1,000, 1 : 3,000, 1 : 5,000, and 1 : 10,000, while the concentration of keratin is 1,000 μg/mL, 100 μg/mL, 10 μg/mL, and 1 μg/mL, respectively. (b) The secondary antibody dilution ratio is 1 : 800, 1 : 1,600, 1 : 3,200, 1 : 5,000, and 1 : 10,000, while the anti-keratin primary antibody dilution ratio is 1 : 200, 1 : 500, 1 : 3,000, and 1 : 5,000, respectively. Each column of the figure represents the mean ± SD (standard deviation) of assays.

(a)

(b)