Development of Simultaneous Analysis of Thirteen Bioactive Compounds in So-Cheong-Ryong-Tang Using UPLC-DAD

Table 2

Precision (intra- and interday) and accuracy of the thirteen analytes.

Analyte concentration (µg/mL)	Intraday ()			Interday ()
	Measured amount (mean ± SD, Dg/mL)	RSD (%)	Accuracy (%)	Measured amount (mean ± SD, Dg/mL)	RSD (%)	Accuracy (%)
Ephedrine
25	5.03 ± 0.02	0.43	100.59	4.97 ± 0.04	0.82	99.30
50	25.59 ± 0.01	0.03	102.36	25.33 ± 0.23	0.90	101.31
100	100.46 ± 0.08	0.08	100.46	100.46 ± 0.31	0.31	100.46
Methyl gallate
2.5	2.48 ± 0.04	1.49	99.36	2.51 ± 0.01	0.35	100.57
12.5	12.29 ± 0.00	0.03	98.34	12.44 ± 0.12	1.00	99.48
50	49.14 ± 0.31	0.63	98.27	50.03 ± 1.00	1.99	100.06
Catechin
2.5	2.53 ± 0.00	0.12	101.09	2.52 ± 0.02	0.75	100.65
12.5	12.50 ± 0.00	0.02	100.00	12.50 ± 0.00	0.02	100.02
50	49.69 ± 0.21	0.43	99.38	50.14 ± 0.48	0.96	100.28
Albiflorin
5	5.02 ± 0.03	0.61	100.36	5.12 ± 0.07	1.42	102.42
25	25.05 ± 0.04	0.15	100.20	25.12 ± 0.11	0.43	100.47
100	98.68 ± 0.16	0.16	98.68	99.78 ± 0.80	0.80	99.78
Paeoniflorin
5	5.05 ± 0.10	1.94	101.10	5.13 ± 0.04	0.73	102.62
25	25.24 ± 0.08	0.30	100.96	25.11 ± 0.27	1.07	100.44
100	101.46 ± 1.96	0.93	101.46	100.46 ± 0.15	0.15	100.46
Benzoic acid
1	1.01 ± 0.01	1.35	100.92	1.01 ± 0.02	1.61	101.28
5	5.02 ± 0.02	0.39	100.31	4.99 ± 0.04	0.80	99.80
100	96.82 ± 2.43	2.51	96.82	100.59 ± 0.28	0.28	100.59
Liquiritin
2.5	2.49 ± 0.01	0.57	99.64	2.44 ± 0.04	1.64	97.67
12.5	12.14 ± 0.01	0.05	97.10	12.37 ± 0.21	1.66	98.99
50	50.20 ± 0.29	0.58	100.39	50.17 ± 0.48	0.96	100.35
Liquiritin apioside
2.5	2.53 ± 0.03	1.08	101.11	2.51 ± 0.00	0.16	100.45
12.5	12.56 ± 0.00	0.02	100.48	12.51 ± 0.04	0.34	100.07
50	49.91 ± 0.36	0.72	99.81	49.95 ± 0.09	0.17	99.90
1,2,3,4,6-Pentagalloyl glucose
5	4.94 ± 0.07	1.45	98.73	5.05 ± 0.03	0.68	101.00
25	25.26 ± 0.02	0.08	101.04	25.15 ± 0.10	0.38	100.60
100	99.89 ± 0.45	0.45	99.89	100.27 ± 0.37	0.37	100.27
Cinnamic acid
0.5	0.50 ± 0.00	0.38	100.16	0.50 ± 0.00	0.33	99.45
12.5	12.50 ± 0.00	0.02	99.96	12.51 ± 0.02	0.13	100.06
50	50.03 ± 0.01	0.02	100.06	50.15 ± 0.19	0.38	100.31
Glycyrrhizin
5	5.03 ± 0.02	0.30	100.62	4.99 ± 0.11	2.27	99.84
25	25.04 ± 0.11	0.42	100.14	25.09 ± 0.01	0.05	100.35
100	100.63 ± 0.30	0.29	100.63	100.65 ± 0.17	0.17	100.65
Schisandrin
1	1.00 ± 0.00	0.11	100.03	1.00 ± 0.00	0.40	100.40
5	5.09 ± 0.00	0.01	101.83	5.04 ± 0.05	0.90	100.89
20	19.33 ± 0.42	2.17	96.64	20.12 ± 0.18	0.87	100.60
6-Shogaol
2	2.04 ± 0.04	2.00	101.93	2.05 ± 0.05	2.60	102.50
50	50.36 ± 0.04	0.09	100.71	50.27 ± 0.18	0.35	100.54
200	197.59 ± 0.09	0.05	98.79	199.67 ± 1.72	0.86	99.83

SD = standard deviation; RSD (%) = (SD/mean) × 100; accuracy (%) = (C_obs/C_nom) × 100.