Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities

Figure 3

(a) HPLC chromatogram of the ethyl acetate extract from A. officinarum Hance; (b) HPLC analysis of the ethyl acetate extract from A. officinarum Hance after spiking with DPPH. Experimental conditions: column, Inertsil-ODS-SP column (150 × 4.6 mm I.D., 5 μm); column temperature, 25°C; mobile phase, 0.2% acetic acid-acetonitrile with the gradient (0–5 min, 55–68% B; 5–15 min, 68% B; 15–30 min, 68–75% B); flow rate, 1.0 mL/min; detection, 254 nm; injection volume, 20 μL.

(a)

(b)