LC-MS/MS Method for Simultaneous Quantification of Dexmedetomidine, Dezocine, and Midazolam in Rat Plasma and Its Application to Their Pharmacokinetic Study

Table 3

The intra- and interday precision and accuracy of dexmedetomidine, dezocine, and midazolam in rat plasma (mean ± SD, 3 days, 6 replicates per day).

Analytes	Spiked conc. (ng/mL)	Intraday ()			Interday ()
		Measured conc. (ng/mL)	Precision (RSD, %)	Accuracy (%)	Measured conc. (ng/mL)	Precision (RSD, %)	Accuracy (%)
Dexmedetomidine	0.1	0.119 ± 0.017	14.28	119.00	0.115 ± 0.015	13.04	115.00
	0.2	0.203 ± 0.022	10.83	101.50	0.197 ± 0.021	10.66	98.50
	5.0	4.63 ± 0.57	12.31	92.60	4.85 ± 0.39	8.04	97.00
	80	78.41 ± 4.95	6.31	98.01	79.36 ± 7.23	9.11	99.20
Dezocine	0.1	0.117 ± 0.015	12.82	117.00	0.114 ± 0.012	10.53	114.00
	0.2	0.206 ± 0.018	8.74	103.00	0.203 ± 0.020	9.85	101.50
	5.0	5.38 ± 0.51	9.48	107.60	5.26 ± 0.42	7.98	105.20
	80	85.15 ± 7.38	8.67	106.43	81.33 ± 5.86	7.21	101.26
Midazolam	0.2	0.226 ± 0.032	14.16	113.00	0.214 ± 0.028	13.08	107.00
	0.4	0.442 ± 0.052	11.76	110.50	0.414 ± 0.038	9.18	103.50
	10.0	10.78 ± 0.714	6.62	107.80	9.88 ± 0.82	8.30	98.80
	160	155.84 ± 13.86	8.89	97.40	159.14 ± 9.42	5.92	99.46