Determination of Curdlan Oligosaccharides with High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection

Table 2

Linearity of the log₁₀ RT versus log₁₀ flow rate (log₁₀ FR) of each single COS separated by HPAEC on a CarboPacPA-100 column.^a

Entry	Compound	Linear equation^b	R^2
1	D-glucose	y = −0.9861x + 0.2611	1.0000
2	(COS)2	y = −0.9894x + 0.3314	1.0000
3	(COS)3	y = −0.9870x + 0.5133	1.0000
4	(COS)4	y = −0.9791x + 0.6612	0.9999
5	(COS)5	y = −0.9789x + 0.8380	0.9998
6	(COS)6	y = −0.9796x + 1.0545	0.9998

^aThe mobile phase was A (water), B (0.2 M sodium hydroxide), and C (0.5 M sodium acetate) with the volume ratio of 50 : 30 : 20; ^by and x refer to the log₁₀ RT and Log₁₀ FR, respectively. The test flow rate range was 0.2, 0.4, 0.6, 0.8, and 1.0 mL/min.