DNA Nanotweezers with Hydrolytic Activity for Enzyme-Free and Sensitive Detection of Fusion Gene via Logic Operation

Figure 1

Characterization of the assembly process of DNA nanotweezer using agarose gel electrophoresis. Lanes 1, 2, 3, and 4 represents strand A, strand B, strand C, and BCR/ABL fusion gene, respectively, and lanes 5 and 6 are strand A + C and strand B + C, lane 7 represents opened nanotweezer assembled from strand A + B + C, and lane 8 represented closed nanotweezer after adding BCR/ABL fusion gene to the system in lane 7. Inset: the images a and b in lanes 7 and 8 represents the nanotweezer-based assay system without and with BCR/ABL fusion gene, respectively.