Multiresidue Method for Quantification of Sulfonamides and Trimethoprim in Tilapia Fillet by Liquid Chromatography Coupled to Quadrupole Time-of-Flight Mass Spectrometry Using QuEChERS for Sample Preparation
Table 2
Validation parameters of sulfonamides and trimethoprim.
Validation parameters
Sulfonamides and trimethoprim
SP
STZ
SMZ
SDMX
SMX
SMPD
SMR
TMP
Working range (ng·g−1)
5–250
5–250
5–250
5–250
5–250
5–250
5–250
5–250
Linearity (R2)
0.9958
0.9914
0.9922
0.9992
0.9994
0.9935
0.9964
0.9984
Sensibility
1174.07
633.811
2324.3
2670.56
1626.52
2057.08
1345.72
1588.92
Matrix effect (%)
12.5 ng·g−1
−18.98
−2.07
−11.19
−3.58
−5.22
9.57
−3.68
9.65
50 ng·g−1
1.60
14.71
0.37
−3.28
1.13
−4.15
1.85
−0.12
100 ng·g−1
0.13
−7.02
0.46
1.61
−1.77
3.74
0.79
−0.03
Accuracy (% recovery (CV%))
10 ng·g−1
83.9 (14.4)
52.9 (19.2)
69.1 (19.4)
49.6 (17.8)
45.4 (11.9)
80.0 (9.4)
79.5 (15.4)
92.0 (13.2)
20 ng·g−1
91.5 (19.3)
38.4 (17.2)
72.4 (11.8)
47.4 (3.4)
41.8 (4.2)
66.8 (9.0)
85.4 (16.7)
88.2 (17.7)
40 ng·g−1
103.6 (19.0)
41.3 (18.6)
68.0 (19.1)
51.1 (4.5)
43.5 (10.0)
64.6 (5.5)
81.2 (15.0)
91.5 (16.2)
LOD (ng·g−1)
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
LOQ (ng·g−1)
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
SP, sulfapyridine; STZ, sulfathiazol; SMZ, sulfamethazine; SDMX, sulfadimethoxine; SMX, sulfamethoxazole; SMPD, sulfamethoxypyridazine; SMR, sulfamerazine; TMP, trimethoprim; LOD, limit of detection; LOQ, limit of quantitation; CV, coefficient of variation.
