Research Article

Rapid Identification and Simultaneous Quantification of Aristolochic Acids by HPLC-DAD and Confirmations by MS in Aristolochia chilensis Using a Limited Biomass

Table 3

Relative percentage for each AA identified present in the extract of Aristolochia chilensis.

Peak numberAAsArea (mAU/min)RT (min)UV-Vis λmax (nm)Relative %

1AAIVa6.45.23221, 242, 251, 293, 329, 41021.1
2AAIa5.75.41224, 254, 320, 39818.9
3AAIII1.96.69251, 284, 314, 3896.3
4AAII0.99.84212, 251, 2993.0
5AAI13.511.72224, 251, 320, 39544.7
6AAIV0.812.57221, 242, 251, 293, 329, 4102.6
Total area30.2