Leakage occurs in a repetitive and consistent manner. (a) The first image without blood leakage in S2 was set as a control condition. Color change shows the amount of leakage. Vessels appear magenta and blood flow is seen with rainbow colors. It is apparent that two disruptive events occurred within 16 min. A much greater amount of leakage was observed during the second disruptive event. (b) Vascular permeability was visually demonstrated, and the graph quantitatively shows about twice as much leakage. The graphical display is consistent with transient leakage from the same site. Endothelial barrier disruption did not always result in leakage. Two arrows in the figure indicate that leakage has occurred. Graph B shows the volume of leakage measured according to site. Scale bar: 10 µm.