Comparison of Three Sample Preparation Procedures for the Quantification of L-Arginine, Asymmetric Dimethylarginine, and Symmetric Dimethylarginine in Human Plasma Using HPLC-FLD

Table 2

Validation data: calibration curve equation, correlation coefficient, LLOQ, ULOQ, intra- and interday precision, and average concentration ± SD quality control samples (human plasma from 6 volunteers) for L-arginine and ADMA using the selected sample preparation (A) with the developed chromatographic method.

	Calibration curve equation	Correlation coefficient (R^2)	LLOQ (µM)	ULOQ (µM)	Intraday precision (CV, %) (n = 6)	Interday precision (CV, %) (n = 9)	Quality control (µM) (n = 6)
L-arginine	y = 78686x − 3519	0.999	0.14	15	3.2	5.0	64.1 ± 10.3
ADMA	y = 73655x + 1134	0.994	0.012	0.50	3.4	3.3	0.27 ± 0.02
SDMA	y = 87003x + 3261	0.989	—	—	4.2	3.8	0.57 ± 0.09