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Journal of Analytical Methods in Chemistry
Volume 2018 (2018), Article ID 6302609, 7 pages
https://doi.org/10.1155/2018/6302609
Research Article

A Molecular Imprinted Polymer as a Flow-Through Optical Sensor for Oxazepam

1División Química Analítica, Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47, Esq. 115, 1900 La Plata, Argentina
2Laboratorio de Investigación y Desarrollo de Métodos Analíticos (LIDMA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47, Esq. 115, 1900 La Plata, Argentina

Correspondence should be addressed to Roberta G. Machicote; ra.ude.plnu.acimiuq@etocihcamr

Received 25 October 2017; Accepted 20 March 2018; Published 4 April 2018

Academic Editor: Krishna K. Verma

Copyright © 2018 Roberta G. Machicote et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A flow-through optosensing system for oxazepam recognition with fluorescence detection was performed by means of a molecular imprinted polymer based on its acid hydrolysis product, 2-amino-5-chlorobenzophenone. The synthesis was conducted via a noncovalent imprinting methodology, using methacrylic acid as a functional monomer and ethylene glycol dimethacrylate as a cross-linking agent. Hydrolysis (types and concentration of acids), polymer retention capacity, binding properties, and elution (selectivity and reversibility) conditions were optimized. The selected molecular imprinted polymer had a molar ratio composition of 1 : 6 : 45 (template : functional monomer : cross-linker). The proposed method was applied to the determination of oxazepam in a pharmaceutical formulation. External standard calibration, standard additions calibration, and Youden’s calibration were carried out in order to evaluate constant and proportional errors due to the matrix. The developed metabolite-based recognition system for benzodiazepines is an innovative procedure that could be followed in routine and quality control assays.