Research Article
Different Spectrophotometric Methods for Simultaneous Determination of Trelagliptin and Its Acid Degradation Product
Table 1
Results obtained by the proposed simultaneous equation spectrophotometric method for determination of TRG and TAD.
| | Item | TRG at 274 nm | TRG at 260.4 nm | TAD at 274 nm | TAD at 260.4 nm |
| | Range of linearity (μg/mL) | 5–50 | 5–50 | 2.5–25 | 2.5–25 | | Regression equation | A = 0.0276 C·μg/mL + 0.0056 | A = 0.0208 C·μg/mL + 0.0063 | A = 0.0129 C·μg/mL–0.0050 | A = 0.0505 C·μg/mL–0.0117 | | Regression coefficient (r) | 0.9999 | 0.9997 | 0.9990 | 0.9999 | | LOD (μg/mL) | 1.01 μg/mL | 1.45 | 0.76 | 0.42 | | LOQ (μg/mL) | 3.05 μg/mL | 4.41 | 2.32 | 1.27 | | 2.16 × 10−4 | 2.35 × 10−4 | 1.54 × 10−4 | 3.28 × 10−4 | | 0.008 | 0.009 | 0.003 | 0.006 | | Confidence limit of the slope | 0.0276 × 5.95 × 10−6 | 0.0208 × 4.89 × 10−6 | 0.0129 × 1.99 × 10−6 | 0.0505 × 1.66 × 10−5 | | Confidence limit of the intercept | 0.0056 × 4.50 × 10−5 | 0.0063 × 5.46 × 10−5 | −0.0050 × 1.42 × 10−5 | −0.0117 × 7.11 × 10−5 | | Standard error of the estimation | 0.009 | 0.008 | 0.003 | 0.006 | Accuracy
Laboratory prepared (mean ± SD) | 100.66 ± 0.10 | 100.96 ± 0.86 | | Standard addition (mean ± SD) | 98.55 ± 1.70 | — | Precision
Intraday % RSD | 0.04–0.53–0.56 | 0.05–0.54–0.55 | | Interday % RSD | 0.40–0.54–0.92 | 0.45–0.52–0.92 | | Drug in dosage form (mean ± SD) | 100.98 ± 0.39 | — |
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Standard deviations of the slope Sb & intercept Sa. |
