Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar Compounds in Traditional Chinese Medicines by HPLC

<div>Separation of the alcohol-soluble extractants of LSW by reverse-phase HPLC using (a) a combinative elution method and (b) a conventional gradient elution method. (c) The blank. The peaks 1, 10, 11, 12, 13, and 14 correspond to 5-hydroxytryptamine, bufotalin, cinobufotalin, bufalin, cinobufagin, and resibufogenin, respectively.</div>

Journal of Analytical Methods in Chemistry

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Figure 1

Figure 1: Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar Compounds in Traditional Chinese Medicines by HPLC 

Figure 1 | Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar Compounds in Traditional Chinese Medicines by HPLC 