Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar Compounds in Traditional Chinese Medicines by HPLC

Table 2

Spike and recovery of 6 components in the ethanol extraction of LSW.

Sample	Low spike (30 µg/mL)	Medium spike (60 µg/mL)	High spike (90 µg/mL)	Mean recovery
5-Hydroxytryptamine	35.34	62.09	86.08	101.2% ± 6.7%
	29.56	60.34	87.29
	30.27	59.80	87.45
Bufotalin	28.93	60.53	95.76	103.2% ± 4.8%
	29.46	65.16	96.45
	29.27	63.61	99.47
Cinobufotalin	28.88	58.58	83.64	94.5% ± 2.9%
	29.84	55.84	82.15
	28.65	56.15	81.78
Bufalin	29.58	61.72	99.41	106.9% ± 3.8%
	32.22	64.59	99.14
	32.50	64.32	98.30
Cinobufagin	30.03	65.27	89.03	104.3% ± 4.9%
	32.41	65.79	88.33
	31.06	65.64	88.82
Resibufogenin	28.43	64.43	91.77	101.1% ± 5.4%
	28.84	64.49	91.27
	28.53	64.59	88.16