Development of an Ultra-High Performance Liquid Chromatography Method for Simultaneous Determination of Six Active Compounds in Fructus aurantii and Rat Plasma and Its Application to a Comparative Pharmacokinetic Study in Rats Administered with Different Doses

Table 3

Intraday and interday precisions and accuracies of meranzin hydrate, naringenin, and hesperetin in rat plasma ().

Components	Spiked concentration (ng/mL)	Intraday			Interday
		Observed concentration (ng/mL)	Precision (RSD, %)	Accuracy (Re, %)	Observed concentration (ng/mL)	Precision (RSD, %)	Accuracy (Re, %)
Meranzin hydrate	6.5	6.30 ± 0.43	6.82	−3	6.28 ± 0.57	9.08	−3.4
	206.0	203.15 ± 5.98	2.94	−1.3	211.84 ± 4.81	2.27	2.8
	3300.0	3290.75 ± 30.54	1.57	−2.5	3293.31 ± 32.25	1.39	−1.2
Naringenin	13.9	13.13 ± 1.64	5.44	−2.5	13.43 ± 1.23	3.66	2.2
	222.0	220.52 ± 6.88	6.71	2.2	219.88 ± 5.11	4.79	2.7
	3555.0	3550.48 ± 33.15	1.36	−1.9	3352.05 ± 35.08	1.22	−1
Hesperetin	3.7	3.44 ± 0.31	9.01	−7	3.64 ± 0.29	7.97	−1.6
	29.5	27.83 ± 2.54	5.50	−3.1	28.12 ± 2.25	5.12	2.4
	236.0	232.23 ± 7.68	3.36	−3	235.64 ± 7.25	2.22	−0.6