Development of an Ultra-High Performance Liquid Chromatography Method for Simultaneous Determination of Six Active Compounds in Fructus aurantii and Rat Plasma and Its Application to a Comparative Pharmacokinetic Study in Rats Administered with Different Doses

Table 4

Extraction recoveries and stability of meranzin hydrate, naringenin, and hesperetin in rat plasma.

Components	Spiked concentration (ng/mL)	Recovery ()		Short-term stability		Freeze-thaw stability		Long-term stability
		Mean ± SD (%)	RSD (%)	Mean ± SD (ng/mL)	RSD (%)	Mean ± SD (ng/mL)	RSD (%)	Mean ± SD (ng/mL)	RSD (%)
Meranzin hydrate	6.5	99.38 ± 1.18	2.01	6.31 ± 0.75	1.18	6.26 ± 0.53	0.85	6.38 ± 1.36	2.13
	206.0	101.81 ± 3.02	2.05	207.84 ± 46.76	2.25	210.11 ± 51.48	2.45	201.56 ± 71.96	3.57
	3300.0	102.01 ± 3.16	2.15	3305.34 ± 335.72	2.24	3310.91 ± 357.30	2.39	3297.28 ± 349.19	3.67
Naringenin	13.9	96.78 ± 1.20	6.05	13.03 ± 2.28	1.20	13.44 ± 4.79	2.74	13.32 ± 5.05	1.87
	222.0	98.25 ± 1.55	2.96	217.05 ± 22.66	2.06	219.51 ± 35.50	2.64	220.06 ± 34.22	2.53
	3555.0	98.96 ± 2.22	1.12	3549.93 ± 455.39	3.35	3552.68 ± 409.74	3.57	3550.10 ± 450.62	3.95
Hesperetin	3.7	96.49 ± 1.42	2.80	3.63 ± 0.87	2.40	3.52 ± 0.43	1.23	3.67 ± 0.57	1.55
	29.5	98.05 ± 2.45	3.03	27.88 ± 3.50	2.61	28.35 ± 4.03	2.71	28.28 ± 1.65	1.15
	236.0	99.17 ± 1.14	2.70	234.18 ± 22.13	3.42	235.03 ± 19.71	3.09	235.66 ± 14.53	2.01