Towards a Dual Lateral Flow Nanobiosensor for Simultaneous Detection of Virus Genotype-Specific PCR Products

Figure 4

Dual lateral flow biosensor assay optimization studies. Representative lateral flow biosensors and signal intensity graphs for (a) oligonucleotide probe amount effect on plasmid pRGNNV amplification product hybridization mixture; (b) oligonucleotide probe amount effect on plasmid pSJNNV amplification product hybridization mixture; (c) hybridization temperature effect on the pRGNNV PCR product and specific oligonucleotide probe hybridization; and (d) hybridization temperature effect on the pSJNNV PCR product and specific oligonucleotide probe hybridization. The signal is visualized with anti-biotin Au NPs. TZ-S: anti-digoxigenin zone; TZ-R: anti-fluorescein zone.

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