Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans

Table 2

Condition optimization. MS intensity for each glycan at three different conditions and the enhancement factors (EFs).

Glycan no.	MS intensity			Enhancement Factor
	Condition 1 MW	Condition 2 SPE	Condition 3 MW + SPE	Con 3/Con 1 w/or w/o SPE	Con 3/Con 2 w/or w/o MW
IS	29738	299037	682825	23.0	2.3
G5	5991	72493	122582	20.5	1.7
G8	2463	9714	12278	5.0	1.3
G10	5328	56814	97849	18.4	1.7
G14	11482	91319	227387	19.8	2.5
G15	2493	11139	15844	6.4	1.4
G17	2604	10991	24068	9.2	2.2
G20	2377	31626	76822	32.3	2.4
G21	13829	93595	270122	19.5	2.9
G23	2372	20225	45111	19.0	2.2
G25	2806	20863	39855	14.2	1.9
G27	5192	32241	83983	16.2	2.6
G28	3974	22301	57710	14.5	2.6
G30	2777	20591	40422	14.6	2.0
Ave ± STD				16.6 ± 7.0	2.1 ± 0.5

We calculated the EFs for the glycans, which were commonly assigned at the three conditions.