Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans

Table 3

MALDI peak intensity table of the assigned glycans in 900–2,000 m/z range.

Glycan ID no.	MS intensity		Ranking	Group	EF
	Method 1 = GT-SPE-DHB/An	Method 2 = DHB/An
G1	22,523	7,904	19	2	2.8
G2	14,247	—	24	3	—
G3	8,621	—	31	3	—
G4	13,752	—	25	3	—
G5	556,350	25,650	2	1	21.7
G6	12,904	—	27	3	—
G7	23,418	—	16	2	—
G8	23,395	—	17	2	—
G9	11,284	—	28	3	—
G10	428,229	18,631	4	1	23.0
G11	8,857	—	30	3	—
G12	14,250	—	23	3	—
G13	38,460	—	13	2	—
G14	495,759	33,830	3	1	14.7
G15	38,971	9,724	12	2	4.0
G16	17,100	6,714	22	3	2.5
G17	78,584	8,961	9	1	8.8
G18	13,420	—	26	3	—
G19	18,481	—	20	2	—
G20	50,126	—	11	2	—
G21	601,498	52,609	1	1	11.4
G22	30,297	10,260	15	2	3.0
G23	81,931	11,867	8	1	6.9
G24	22,814	8,421	18	2	2.7
G25	89,870	10,485	7	1	8.6
G26	18,283	—	21	3	—
G27	205,902	23,231	5	1	8.9
G28	105,748	14,170	6	1	7.5
G29	9,020	—	29	3	—
G30	52,571	8,941	10	1	5.9
G31	30,831	—	14	2	—
Ave ± STD					8.8 ± 6.5

Method 1 = GT-SPE-DHB/An method; Method 2 = DHB/An method. Group 1 = ranking scale from 1 to 10; Group 2 = from 11 to 20; Group 3 = from 21 to 31. EF = peak intensity of Method 1 ÷ peak intensity of Method 2.