One-Step Solid Extraction for Simultaneous Determination of Eleven Commonly Used Anticancer Drugs and One Active Metabolite in Human Plasma by HPLC-MS/MS

Table 2

Recovery of all the analytes after extraction with different extraction solvents.

Extraction solvent	Paclitaxel (%)	Docetaxel (%)	Vinblastine (%)	Vinorelbine (%)	Pemetrexed (%)	Carboplatin (%)	Etoposide (%)	Cyclophosphamide (%)	Ifosfamide (%)	Gemcitabine (%)	Irinotecan (%)	SN-38 (%)
Methanol	ND	ND	ND	ND	40.6	50.3	54.4	51.9	45.4	56.3	56.2	65.1
Acetonitrile	24.8	48.8	35.3	44.3	38.2	48.2	57.3	49.5	47.2	57.5	62.8	60.9
Ethyl acetate	53.4	35.3	40.6	57.5	ND	ND	88.0	74.6	71.3	85.3	64.3	68.4
Diethyl ether	93.3	46.9	47.0	82.3	ND	ND	80.4	65.3	63.9	ND	64.0	70.7
tert-Butyl methyl ether	76.3	37.0	39.4	68.9	ND	ND	32.2	67.2	67.3	ND	29.3	35.3
Ether-dichloromethane (8 : 2)	80.6	30.9	43.3	88.4	ND	ND	42.7	68.0	66.7	ND	56.7	60.8
Ether-dichloromethane (7 : 3)	52.2	74.5	65.7	80.3	ND	ND	82.4	69.5	60.3	ND	63.1	59.5
Ether-dichloromethane (5 : 5)	69.1	48.4	58.0	67.8	ND	ND	75.9	67.3	59.7	ND	60.7	63.3
Oasis HLB 1 cc (10 mg)	64.0	58.8	56.5	72.3	ND	ND	84.3	91.9	86.4	48.2	82.0	75.8
Ostro 96-well plate	62.4	86.3	90.4	71.2	74.4	73.9	64.7	99.1	77.7	71.4	75.4	68.5