One-Step Solid Extraction for Simultaneous Determination of Eleven Commonly Used Anticancer Drugs and One Active Metabolite in Human Plasma by HPLC-MS/MS

Table 6

Stability of analytes in human plasma ().

Analyte	Nominal concentration (ng/mL)	Three freeze-thaw stability		Short-term stability (24 h)		Long-term stability (3 months)
		Precision (%RSD)	Accuracy (%RE)	Precision (%RSD)	Accuracy (%RE)	Precision (%RSD)	Accuracy (%RE)
Paclitaxel	50.0	1.9	5.9	2.8	6.7	2.4	5.8
	250.0	1.9	3.9	5.6	−1.4	3.6	3.7
	1250.0	4.3	−5.5	4.0	−4.7	3.3	−1.0
Docetaxel	20.0	1.8	6.8	3.1	4.5	2.5	5.3
	100.0	2.1	6.9	4.1	1.6	2.2	3.3
	500.0	1.9	−4.6	2.5	−3.1	2.9	−7.3
SN-38	20.0	3.6	−4.7	2.8	2.6	4.6	−3.1
	100.0	2.9	4.5	4.6	−3.0	3.5	2.9
	500.0	4.1	1.6	3.8	2.7	6.2	3.8
Vinorelbine	200.0	2.0	8.1	3.6	2.8	4.2	6.8
	1000.0	7.2	1.3	4.2	−0.4	6.3	0.9
	5000.0	5.4	3.0	3.3	4.9	3.2	3.5
Pemetrexed	200.0	5.0	2.6	4.7	4.8	4.9	5.2
	1000.0	2.8	2.7	6.5	−1.8	2.4	2.0
	5000.0	3.5	−6.4	4.8	−5.1	4.7	−4.7
Vinblastine	50.0	1.4	0.2	1.9	2.0	4.2	5.1
	500.0	5.0	−2.9	5.5	−3.1	4.0	3.0
	5000.0	3.5	1.5	3.9	2.9	3.2	3.3
Irinotecan	50.0	3.8	5.6	2.7	3.8	3.6	5.6
	500.0	5.5	−3.1	7.4	−1.0	4.5	2.8
	5000.0	2.8	4.3	4.4	1.3	4.0	3.9
Cyclophosphamide	5.0	7.4	1.2	1.9	4.3	5.5	−0.1
	50.0	6.1	3.7	3.0	2.8	3.6	4.8
	500.0	1.8	−4.2	2.4	−4.2	2.1	−3.7
Ifosfamide	5.0	3.5	2.4	2.6	0.2	5.3	3.5
	50.0	4.5	4.2	6.0	3.1	4.6	2.7
	500.0	3.2	−0.2	3.1	0.9	3.5	0.2
Carboplatin	100.0	7.2	4.8	6.9	−1.4	6.2	0.8
	500.0	6.7	2.6	6.6	1.0	7.4	3.1
	2500.0	1.7	−3.0	2.3	−0.7	2.1	−0.9
Etoposide	100.0	4.7	2.7	5.8	0.2	4.7	−0.3
	500.0	3.4	4.4	3.6	−2.5	4.5	3.5
	2500.0	7.3	−1.2	5.9	−4.1	6.5	−2.8
Gemcitabine	100.0	6.7	−1.4	5.6	−2.3	2.5	5.3
	500.0	7.8	−1.3	7.2	−2.0	6.7	−3.2
	2500.0	2.4	−7.2	5.4	−5.1	4.9	−4.0