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Journal of Analytical Methods in Chemistry
Volume 2018, Article ID 8034042, 8 pages
Research Article

Determination of Cyclopropane Fatty Acids in Food of Animal Origin by 1H NMR

Department of Food and Drug, University of Parma, Parco Area delle Scienze 27A, 43124 Parma, Italy

Correspondence should be addressed to Augusta Caligiani; ti.rpinu@inaigilac.atsugua

Received 31 July 2017; Revised 11 October 2017; Accepted 12 February 2018; Published 1 April 2018

Academic Editor: Mercedes G. Lopez

Copyright © 2018 Veronica Lolli et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Cyclopropane fatty acids (CPFAs) are unusual fatty acids of microbial origin, recently detected in milk and dairy products. CPFAs have been demonstrated to be interesting molecular markers for authentication of dairy products obtained without ensiled feeds. Moreover, they can also be recognized as a new secondary component of human diet. Information is lacking on the presence of cyclic fatty acids in other food sources. Cyclopropane fatty acids have been detected by GC-MS analysis in cheese and other animal fats in concentration ranging from 200 to 1000 mg/kg fat, but in some cases, the complex fatty acid profile and the possible presence of interfering peaks make the separation not straightforward and the quantification uneasy. Therefore, a new reliable 1H NMR method was developed to detect and measure CPFA content in different foods of animal origin, based on the detection of the characteristic signals of cyclopropane ring. The 1H NMR (600 MHz) method showed detection limits comparable with those of full scan GC-MS, and it allowed the identification and quantitation of the cyclopropane fatty acids in different foods.