Sensitive Fluorescent Sensor for Recognition of HIV-1 dsDNA by Using Glucose Oxidase and Triplex DNA

Figure 4

(a) Effects of capture DNA concentrations; (b) effects of signal DNA concentrations; (c) effects of spermine concentrations; (d) effects of streptavidin concentrations; (e) effects of biotin-GOx concentrations; (f) effects of HPPA concentrations; (g) effects of HRP concentrations; (h) effects of time of hybridization. When each of these factors is optimized, the other factors maintain optimal conditions (1.4 μM capture DNA, 50 nM signal DNA, 120 μM spermine, 800 ng·mL⁻¹ SA, 50 μg·mL⁻¹ biotin-GOx, 200 μM HPPA, 20 ng·mL⁻¹ HRP, and 60 min of time of hybridization).

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