Research Article
Calorimetric Study of Helix aspersa Maxima Hemocyanin Isoforms
Table 2
Arrhenius equation parameters, estimated for the two-state irreversible model of the thermal denaturation of isoform αD+N-HaH, at neutral pH.
| | Parameter | Temperature scan rate (°C min−1) | | 0.2 | 0.5 | 1.0 | 1.5 |
| | First transition | | Tm (°C) | 73.4 | 74.0 | 76.4 | 77.0 | | ΔHcal (MJ·mol−1) | 31.10 | 34.07 | 39.10 | 41.27 | | Ea (kJ·mol−1) | 424 ± 9 | 434 ± 6 | 418 ± 5 | 423 ± 7 | | (°C) | 80.8 ± 0.5 | 79.0 ± 0.3 | 80.0 ± 0.4 | 79.0 ± 0.3 | | r | 0.989 | 0.985 | 0.988 | 0.987 | | Second transition | | Tm (°C) | 77.1 | 79.02 | 80.07 | 81.20 | | ΔHcal (MJ·mol−1) | 69.9 | 79.0 | 79.8 | 81.4 | | Ea (kJ·mol−1) | 586 ± 12 | 564 ± 8 | 596 ± 10 | 601 ± 14 | | (°C) | 83.8 | 83.9 | 83.7 | 83.6 | | r | 0.995 | 0.996 | 0.993 | 0.994 |
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