Research Article
Rapid Screening and Quantitative Determination of Active Components in Qing-Hua-Yu-Re-Formula Using UHPLC-Q-TOF/MS and HPLC-UV
Table 2
Linear regression data, LODs, and LOQs of six compounds.
| | Components | Regression equations | R2 | Linear range (μg/mL) | LODs (μg/mL) | LOQs (μg/mL) |
| | Danshensu | y = 5508.1x + 5.404 | 0.9999 | 2.6–500 | 0.7 | 2.6 | | Paeoniflorin | y = 419.45x − 6.7219 | 0.9994 | 20.8–25,000 | 5.0 | 20.8 | | Acteoside | y = 3283.3x − 11.072 | 0.9997 | 5.2–1000 | 1.0 | 5.2 | | Lithospermic acid | y = 12900x − 123.54 | 0.9991 | 10.4–1000 | 0.6 | 10.4 | | Salvianolic acid B | y = 1616.6x − 9.4854 | 0.9993 | 5.2–10,000 | 2.0 | 5.2 | | Salvianolic acid C | y = 19071x + 88.12 | 1.0000 | 10.4–1000 | 0.8 | 10.4 |
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y = Ax + B; y is the peak area; x is the concentration of the analytes; R2 is the correlation coefficient of the equation.
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