Rapid Screening and Quantitative Determination of Active Components in Qing-Hua-Yu-Re-Formula Using UHPLC-Q-TOF/MS and HPLC-UV

Table 4

Recovery of six compounds in QHYRF ().

Components	Contents	Quantity added	Theoretical amount	Recorded amount	Recovery	RSD
	(mg/mL)	(mg/mL)	(mg/mL)	(mg/mL)	(%)	(%)
Danshensu	0.22	0.05	0.27	0.27	100.6	1.7
		0.11	0.33	0.33	100.0	3.3
		0.2	0.42	0.42	100.2	1.8
Paeoniflorin	12.32	2.45	14.77	14.78	100.4	0.4
		4.95	17.27	17.27	100.0	2
		7.85	20.17	20.17	100.0	5.4
Acteoside	0.07	0.15	0.22	0.22	103.1	3.7
		0.22	0.29	0.29	99.0	5.5
		0.42	0.49	0.49	100.6	5.3
Lithospermic acid	0.1	0.08	0.18	0.18	102.6	4.3
		0.15	0.25	0.25	103.3	5.4
		0.27	0.37	0.38	102.2	1.7
Salvianolic acid B	4.96	1	5.96	5.96	99.9	3.7
		2.23	7.19	7.19	100.0	3.5
		3.41	8.37	8.37	100.1	2.2
Salvianolic acid C	0.01	0.09	0.1	0.10	103.6	3
		0.21	0.22	0.22	99.0	2.3
		0.35	0.36	0.38	104.7	4.8