A Localized Surface Plasmon Resonance Sensing Method for Simultaneous Determination of Atenolol and Amiloride in Pharmaceutical Dosage Forms and Urine Samples

Table 1

Statistical parameters of the proposed method.

Statistical parameter	Atenolol	Amiloride
Linearity range	1 × 10^−5–1 × 10^−4 mol·L^−1	1 × 10^−6–1 × 10^−5 mol·L^−1
Intercept ± SD^a	0.34 ± 0.0055	0.019 ± 0.0038
Slope ± SD^b	0.027 ± 9.3 × 10^−4	0.039 ± 6.4 × 10^−4
R^2c	0.9941	0.9986
R^d	0.9970	0.9993
Mandel test value^e	7.8 × 10^−9	7.2 × 10^−8
LOD (mol·L^−1)^f	0.89 × 10^−5	0.42 × 10^−6
LOQ (mol·L^−1)^g	2.71 × 10^−5	1.28 × 10^−6
Sy^h	0.0072	0.0050
Sx0^i	0.0862	0.125
RSD (n = 3)^j	0.178	0.565
Intraday precision^k	1.94–2.78	1.01–1.67
Interday precision^k	1.64–3.10	1.25–2.40

^aStandard deviation of intercept, ^bstandard deviation of slope, ^cdetermination coefficient, ^dcorrelation coefficient, ^etabulated F values at is 7.7 at (df1 (1), df2 (n-3) = (1,4)), ^flimit of detection, ^glimit of quantitation, ^hstandard error, ⁱmethod standard deviation, ^jrelative standard deviation, and ^kaverage of six determinations at three different concentration levels.