Development of Micellar HPLC-UV Method for Determination of Pharmaceuticals in Water Samples

Figure 1

Influence of the nature of the organic modifier in the analyte separation. Solvents: (a) n-butanol, (b) n-propanol, and (c) ethanol. Data were obtained for standard solution of amoxicillin (1), caffeine (2), tetracycline (3), diclofenac (4), ibuprofen (5), 17α-ethynylestradiol (6), levonorgestrel (7), nimesulide (8), ciprofloxacin (9), and norfloxacin (10) in the concentration of 20.0 mg·L⁻¹, with a injected volume of 20 μL, at a flow rate of 1.0 mL·min⁻¹, at 25°C, and detection at 220 nm. Phase A with 3.0% (v/v) of solvent and 0.3% (m/v) SDS and phase B with 15% (v/v) of solvent and 3.0% (m/v) SDS and both with 20 mmol·L⁻¹ of phosphate at pH 7.0. The mobile phase was changed from 100% of phase A to 100% of phase B in 30 min. A in the y-axis is the absorbance.